Table 6

RT-qPCR analysis of the WT as well as the cprR and parR mutants under different conditions

Strains and conditionsaFold change in expression of the indicated gene
arnBpmrBparRcprR
WT plus CP28/WT335.6 ± 30.421.6 ± 1.21.9 ± 0.10.8 ± 0.1
WT plus CP28/cprR mutant plus CP2858.1 ± 30.824.4 ± 1.2NTbNT
WT plus CP28/parR mutant plus CP281.1 ± 0.30.8 ± 0.1NTNT
WT plus indolicidin/WT150.0 ± 50.115.19 ± 1.91.9 ± 0.21.6 ± 0.1
WT plus indolicidin/cprR mutant plus indolicidin4.9 ± 2.61.34 ± 0.1NTNT
  • a Grown in BM2 medium with or without indolicidin and grown in BM2 and then exposed to 12 μg/ml CP28 for 30 min.

  • b NT, not tested.