Table 4

MICs of antibiotics (μg/ml), biocides (μg/ml), and tea tree oil and terpinen-4-ol (%, vol/vol) for three Staphylococcus strains serially subcultured with terpinen-4-ol or tea tree oila

AgentS. aureus NCTC 6571S. aureus ATCC 25923S. epidermidis ATCC 12228
Passage no.MICPassage no.MICPassage no.MIC
ControlWith 0.1% T4olControlWith 0.1% T4olWith 0.1% TTOControlWith 0.2% T4ol
AMX190.120.06180.120.120.121410.5
220.250.25200.50.251710.5
CIP170.250.06160.120.120.25140.250.25
190.120.06180.250.060.12170.50.25
220.120.06200.250.06
GEN1712160.50.250.5140.120.5
1911180.250.120.12170.250.25
2212200.250.25
TET170.120.06160.120.120.25140.50.5
190.25<0.03180.250.120.251710.5
220.120.06200.250.12
VAN1711162221444
1910.5181121744
220.50.252011
Benzalkonium170.511620.521421
Chloride19111820.511721
22112020.5
Triclosan190.060.03180.060.030.03170.030.06
220.120.12200.250.12
Tea tree oil170.50.25160.120.250.25140.50.5
1910.5180.50.50.5170.50.5
220.250.25200.50.5
Terpinen-4-ol170.250.5160.250.50.5140.50.5
190.50.5180.50.50.25170.50.5
220.120.25200.120.25
  • a Boldface type indicates a difference in MIC of 4-fold or more for passaged and nonpassaged strains.