Table 1

Characteristics of K. pneumoniae strain BK32529 and the Tn4401d-harboring E. coli DH10 transformant

IsolateInC type(s)aβ-LactamasesbMIC (mg/liter)c
IMPMERERTDORCAZCTXCEFATMAMIGENTOBTIC-CLAVPIP-TAZCIPLEVSXTTGCCOLPLB
BK32529A/C, FIIsTEM-1, SHV-12≤12>42>16>32>16>16>32>8>8>128/2>64/4>2>8>4/761>4>4
DH10B transformantA/CTEM-1, SHV-12≤1≤1≤0.25≤0.12>164≤2>16>328>864/2<8/4≤0.25≤1>4/760.50.50.5
  • a Plasmid incompatibility (InC) groups were determined using the multiplex-PCR method described by Carattoli et al. (2).

  • b blaCTX-M, blaSHV, blaTEM, blaGES, blaNDM, blaVIM, blaIMP, blaOXA-48, blaBIC, blaSPM, blaGIM, blaAIM, blaSIM, and blaDIM, as well as AmpC β-lactamases blaACT-1, blaACC, blaBIL-1, blaCMY, blaDHA, blaFOX, blaLAT, blaMIR-1, and blaMOX, were identified by PCR using methods described elsewhere (8, 18).

  • c MICs were determined using broth microdilution; resistance is indicated in boldface. IMP, imipenem; MER, meropenem; ERT, ertapenem; DOR, doripenem; CAZ, ceftazidime; CTX, cefotaxime; CEF, cefepime; ATM, aztreonam; AMI, amikacin; GEN, gentamicin; TOB, tobramycin; TIC-CLAV, ticarcillin-clavulanate; PIP-TAZ, piperacillin-tazobactam; CIP, ciprofloxacin; LEV, levofloxacin; SXT, trimethoprim-sulfamethoxazole; TGC, tigecycline; COL, colistin; PLB, polymyxin B.