Table 2

blaKPC excision patterns in additional Tn4401-bearing strains

StrainSpeciesInC type(s)aMLSTbKPCTn4401 variantcSize(s) of Tn4401 deletion pattern(s) (bp)d
1K. pneumoniaeN, FIIs63KPC-4b160, 278, 430
2K. pneumoniaeUnk76KPC-3a155, 217
3K. pneumoniaeFIIs234KPC-2a289
4K. pneumoniaeFIIs258KPC-2a278, 430
5K. pneumoniaeFIIs258KPC-3b186, 278, 430
6K. pneumoniaeN, A/C258KPC-2a215, 278, 430
7K. pneumoniaeUnk258KPC-2a191, 278, 430
8K. pneumoniaeFIIs258KPC-3b278, 430
9K. pneumoniaeFIIs258KPC-3−68255
10K. pneumoniaeA/C486KPC-2b278, 430
11E. coliL1, N, Y, FIA, FIB, FIIs2KPC-2b278, 430
12E. coliL1, N, Y, FIA, FIB, FIIs2KPC-3b278, 430
13E. coliL1, FIA, FIB, FIIs43KPC-2a278, 430
14E. coliFIB, A/C, Frep33KPC-2a278, 430
15E. coliA/C35KPC-3b278
  • a Plasmid replicon typing was performed using a method previously described by Carattoli et al. (2). Unk, unknown.

  • b MLST for K. pneumoniae was performed using the method described by Diancourt et al. (9); MLST for E. coli was performed using the Institut Pasteur MLST scheme (http://www.pasteur.fr/recherche/genopole/PF8/mlst/EColi.html).

  • c Tn4401 variants were identified by PCR (6), followed by DNA sequencing. “−68” indicates the previously described variant with a 68-bp deletion upstream of blaKPC (13).

  • d Tn4401 deletion patterns were identified by nested PCR and are expressed as the PCR product length resulting from amplification with primer set F2/R2.