Table 1

Sensitivity of HIV-1BaL (or strain C3 where indicated) to saquinavir in various cell and tissue culture systems

Cell/tissue culture systemExposure to drugEC (μM)a
50%90%
PulseSustainedPulseSustained
PM-1 cell lineWith virus1.16 (0.37)0.03 (0.01)15.15 (13.84)0.12 (0.04)
4 h after virus0.46 (0.35)0.05 (0.03)4.03 (3.23)1.07 (0.88)
24 h after virus0.62 (0.02)0.11 (0.09)2.98 (0.17)0.51 (0.42)
PBMCs (BaL)With virus1.51 (2.62)0.003 (0.005)3.54 (4.96)0.006 (0.007)
PBMCs (C3)With virus1.05 (1.38)NA2.10 (1.48)NA
MDMs (BaL)With virus13.92 (7.71)0.06 (0.02)71.36 (74.10)0.85 (0.62)
MDMs (C3)With virus0.10 (0.14)*NA0.53 (0.76)*NA
iMDDCs (direct infection)With virus0.20 (0.01)*NA2.19 (0.21)*NA
iMDDCs (trans infection)With virus1.12 (0.29)NA1.64 (0.59)NA
Infectious virus from cervical tissueWith virus19.31 (20.28)0.07 (0.11)42.83 (60.08)0.23 (0.31)
Infectious virus from penile tissueWith virus4.68 (4.42)0.04 (0.03)7.21 (4.30)0.06 (0.04)
Infectious virus from colorectal tissueWith virus3.78 (4.08)NA6.41 (6.07)NA
24 h after virus6.53 (3.51)NA13.75 (3.25)NA
48 h after virus15.32 (8.47)NA30.05 (24.64)NA
Direct infection of colorectal tissueWith virus0.72 (0.97)NA2.06 (1.54)NA
Cervical migratory cells and T cellsWith virus4.32 (7.00)2.35 (4.02)11.95 (8.68)5.71 (9.76)
Penile migratory cells and T cellsWith virus13.54 (8.12)1.56 (1.03)44.52 (47.73)2.12 (1.32)
Colorectal culture supernatants and T cellsWith virus4.15 (2.48)NA30.27 (24.12)NA
  • a The 50% and 90% effective concentrations shown are the means and standard deviations (in parentheses) derived from a minimum of three independent experiments performed in triplicate; values marked with an asterisk are derived from two independent experiments performed in triplicate. NA, not applicable.