Table 1

List of strains used in experiments

StrainRelevant genotype and phenotypeaReference or source
E. coli strains
    AG100E. coli wt strain11
    AG100/pACrarA-1AG100 + pACrarA-1 (wt rarA cloned into pACYC177 (BamHI, ScaI), KanrThis work
    AG100/pACYC177AG100 + pACYC177 (Kanr, Ampr)This work
    AG100/pACrarA-2AG100 + pACrarA-2 (wt rarA cloned into pACYC184 [BamHI, HindIII]), CmrThis work
    AG100/pACYC184AG100 + pACYC184 (Cmr, Tetr)This work
    AG100AacrAB efflux pump-deleted strain28
    AG100A/pACrarA-2AG100A + pACrarA-2 (wt rarA cloned into pACYC184 [BamHI, HindIII]), CmrThis work
    AG100A/pACYC184AG100A + pACYC184 (Cmr, Tetr)This work
    MG1655E. coli K-12 laboratory isolateIn-house strain collection
    MG1655/pACrarA-1MG1655 + pACrarA-1 (wt rarA cloned into pACYC177 [BamHI, ScaI]), KanrThis work
    MG1655/pACYC177MG1655 + pACYC177 (Kanr, Ampr)This work
    MG1655 ΔmarAmarA-deleted strainS. B. Levy, L. McMurry
    MG1655 ΔmarA/pACrarA-1MG1655 ΔmarA + pACrarA-1 (wt rarA cloned into pACYC177 [BamHI, ScaI]), KanrThis work
    MG1655 ΔmarA/pACYC177MG1655 ΔmarA + pACYC177 (Kanr, Ampr)This work
    AG100 ΔsoxS Δrob ΔmarAsoxS-, rob-, marA-deleted strainS. B. Levy, L. McMurry
    AG100 ΔsoxS Δrob ΔmarA/pACrarA-2AG100 ΔsoxS Δrob ΔmarA + pACrarA-2 (wt rarA cloned into pACYC184 [BamHI, HindIII]), CmrThis work
    AG100 ΔsoxS Δrob ΔmarA/pACYC184AG100 ΔsoxS Δrob ΔmarA + pACYC184 (Cmr, Tetr)This work
K. pneumoniae strains
    MGH 78578ATCC 70072119
    Ecl8K. pneumoniae wt strain8
    Ecl8/pACrarA-1Ecl8 + pACrarA-1 (wt rarA cloned into pACYC177 [BamHI, ScaI]), KanrThis work
    Ecl8/pACYC177Ecl8 + pACYC177 (Kanr, Ampr)This work
    Ecl8Mdr1Spontaneous MDR mutant of Ecl810
    Ecl8 ΔramAramA-deleted strain derived from Ecl827
    Ecl8 ΔramA/pACrarA-1Ecl8 ΔramA + pACrarA-1 (wt rarA cloned into pACYC177 [BamHI, ScaI]), KanrThis work
    Ecl8 ΔramA/pACYC177Ecl8 ΔramA + pACYC177 (Kanr, Ampr)This work
    Kp342K. pneumoniae isolated from maize9
    Ecl8 ΔacrABacrAB-deleted strain derived from Ecl8, KanrThis work
    Ecl8 ΔacrAB/pACrarA-2Ecl8 ΔacrAB + pACrarA-2 (wt rarA cloned into pACYC184 [BamHI, HindIII]), CmrThis work
    Ecl8 ΔacrAB/pACYC184Ecl8 ΔacrAB + pACYC184 (Cmr, Tetr)This work
    Ecl8 ΔrarArarA (KPN_02968)-deleted strain derived from Ecl8, KanrThis work
    Ecl8 ΔrarA/pACrarA-2Ecl8 ΔrarA + pACrarA-2 (wt rarA cloned into pACYC184 [BamHI, HindIII]), CmrThis work
    Ecl8 ΔrarA/pACYC184Ecl8 ΔrarA + pACYC184 (Cmr, Tetr)This work
    TS152, TS165K. pneumoniae clinical isolates (Turkey)27
    TS180, TS202K. pneumoniae clinical isolates (Chile)27
    GC9, GC12, GC19, GC21K. pneumoniae clinical isolate (Germany)This work
    GC9, GC12, GC19, GC21/pACoqxRGC isolates + pACoqxR (wt oqxR cloned into pACYC177 [BamHI, ScaI]), KanrThis work
    GC9, GC12, GC19, GC21/pACYC177GC isolates + pACYC177 (Kanr, Ampr)This work
  • a wt, wild type; Kan, kanamycin; Amp, ampicillin; Cm, chloramphenicol; Tet, tetracycline.