Table 3

Susceptibility profiles of E. coli strains transformed with pACrarA and vector-only controla

StrainMIC (μg/ml)
CHLOQXTETTIGNORCIP
AG1004164< 0.5000.1250.031
AG100/pACrarA-116328210.062
AG100/pACYC1774164< 0.5000.1250.031
AG100/pACrarA-2*32*20.2500.062
AG100/pACYC184*16*< 0.50.1250.031
AG100A*8*0.2500.0080.004
AG100A/pACrarA-2*8*0.2500.0080.004
AG100A/pACYC184*8*0.2500.0080.004
AG100 ΔsoxS Δrob ΔmarA*16*0.5000.0320.031
AG100 ΔsoxS Δrob ΔmarA/pACrarA-2*32*40.2500.062
AG100 ΔsoxS Δrob ΔmarA/pACYC184*16*0.5000.0310.031
MG1655416440.5000.062
MG1655/pACrarA-116328810.125
MG1655/pACYC177416440.5000.062
MG1655 ΔmarA48440.2500.031
MG1655 ΔmarA/pACrarA-1321616810.125
MG1655 ΔmarA/pACYC17748440.2500.031
  • a A pACYC177 or pACYC184 backbone was used to clone the rarA open reading frame. Depending on the resistance cassettes already in situ, the appropriate recombinant constructs would be used. Asterisks denote that MIC testing for that particular antibiotic was not done due to presence of a conflicting antibiotic resistance cassette on the plasmid and/or construct. Entries in bold denote increased MICs over those seen with wild-type/parental strains. CHL, chloramphenicol, OQX, olaquindox, TET, tetracycline, TIG, tigecycline, NOR, norfloxacin, CIP, ciprofloxacin.