Table 2

List of plasmids and strains used for cloning purposes

Strain or plasmidRelevant characteristicsReference
E. coli CC118Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recAl5
P. aeruginosa PAO17
pME6031PVS1-p15A shuttle vector; Tcr4
pMES-23SA 5.95-kb SacI-PstI fragment containing the rRNA operon from CF30-1979 inserted into pME6031This study
pMES-23S(A2045G)A 5.95-kb SacI-PstI fragment containing the rRNA operon from CF333-2007 inserted into pME6031This study
pMES-23S(C2598T)A 5.95-kb SacI-PstI fragment containing the rRNA operon from CF223-2002 inserted into pME6031This study