Table 4

Relative expression and sequence of PBP2 and PBP3 genes from 3 pairs of clonally related susceptible-PBLR sequential P. aeruginosa isolatesa

StrainPBP2 gene expressionbPBP2 sequencecPBP3 gene expressionPBP3 sequence
SusceptiblePBLRSusceptiblePBLR
PAO11N/AWT1N/AWT
P82.69 ± 1.894.63 ± 1.43WT0.57 ± 0.040.60 ± 0.33WT
P140.99 ± 0.271.01 ± 0.73WT0.67 ± 0.310.49 ± 0.23L3V
P161.01 ± 0.731.95 ± 0.11WT0.33 ± 0.070.75 ± 0.27WT
  • a N/A, not applicable; WT, wild type.

  • b Relative mRNA expression compared to that of wild-type PAO1.

  • c Sequence compared to that of wild-type PAO1. In all cases, the susceptible and the PBLR isolates from each pair yielded an identical sequence.