Table 1

Inhibition of alpha herpesvirus (HSV-1), betaherpesvirus (HCMV), and gammaherpesvirus (EBV) by CGsa

VirusMean EC50 (μM) ± SDMean CC50 (μM) ± SDSIHost cell
DigoxinOuabainDigoxinOuabainDigoxinOuabain
HCMV0.036 ± 0.000.017 ± 0.000.45 ± 0.110.10 ± 0.0212.66.08HFF
HCMV0.086 ± 0.010.021 ± 0.000.48 ± 0.240.21 ± 0.075.610U373
HSV-10.10 ± 0.010.026 ± 0.000.89 ± 0.100.58 ± 0.018.922Vero
EBV0.15 ± 0.000.077 ± 0.010.217 ± 0.050.10 ± 0.001.451.31Akata
  • a The EC50, CC50, and selectivity index (SI) values of digoxin and ouabain against HCMV, HSV-1, and EBV in different cell lines are shown. The EC50 of digoxin against HCMV in HFF cells has been reported to be 0.02 μM (13). The EC90s of digoxin and ouabain against HSV-1 in Vero cells were 0.15 and 0.04 μM, respectively (7). Mean values of triplicate determinations from three independent experiments are shown.