Table 3

ORFs derepressed in vraS and vraT mutantsa

Fold repression by Ox in wtFold derepression in mutantsaORF descriptionFunctionS. aureus USA300 locusLocus
923ΔvraT923vraS
6.45.42.5Protein A, SpaPathogenesisSAUSA300_0113SA0107
2.12.52.2SirA, iron ABC transporter, lipoproteinTransporterSAUSA300_0117SA0111
22.41.9Branched-chain amino acid transport proteinAmino acid transportSAUSA300_0188SA0180
3.22.32.9AcpD, FMN-dependent NADH azoreductasesynthesis of membrane-derived oligosaccharides from UDP-glucoseSAUSA300_0206SA0204
3.73.42Antiholin-like protein LrgAPeptidoglycan degradationSAUSA300_0256SA0252
2.92.61.8Antiholin-like protein LrgBPeptidoglycan degradationSAUSA300_0257SA0253
4.43.72.6Hypothetical proteinHypotheticalSAUSA300_0274SA0269
2.32.62.1Hypothetical proteinHypotheticalSAUSA300_0275NF
3.92.42.8Staphyloxanthin biosynthesis proteinTransport and pathogenesisSAUSA300_0277SA0270
2.42.32.5EsxATransport and pathogenesisSAUSA300_0278SA0271
2.52.53.4EsaATransport and pathogenesisSAUSA300_0279SA0272
2.82.32.2Acid phosphatase 5′-nucleotidase, lipoproteinDegradation, transportSAUSA300_0307SA0295
2.54.14.4Superoxide dismutase (Mn/Fe family)Stress responseSAUSA300_0314SA0303
2.32.82.5HypotheticalHypotheticalSAUSA300_0938NF
2.22.42.2Glycosyltransferase, group 1 family proteinStarch/sucrose metabolismSAUSA300_0939SA0522
2.52.92.6Putative lipoproteinLipoproteinSAUSA300_1106SA1056
2.32.21.8HypotheticalHypotheticalSAUSA300_1210NF
6.83.65.1ThermonucleaseEndoribonuclease, pathogenesisSAUSA300_1222SA1160
3.222.3Probable nitroreductase family proteinNitrate/nitrite metabolismSAUSA300_1986SA1840
2.82.11.9ABC transporter NatATransporterSAUSA300_2288SA2132
2.62.22.2HypotheticalHypotheticalSAUSA300_2289SA2133
9.63.12.6ABC transporterTransporterSAUSA300_2453SA2302
6.32.31.9Membrane-spanning protein SmpBMembrane spanningSAUSA300_2454SA2303
2.22.42.3HypotheticalHypotheticalSAUSA300_2528SA2377
4.33.53.6Repressor of toxins RotRegulation, pathogenesisSAUSA300_2543SA2403
2.22.72.5Citrate transporterTransporter, metabolismSAUSA300_2552SA2411
  • a Strains were compared with the wild type in the presence of Ox. ORFs are listed in the ascending order of the USA300 locus number. The SA prefix indicates that the annotation is from the genome sequence of strain N315; the USA300 prefix is from the sequence of USA300 strain FPR3757. NF, not found.