Table 3

Alterations of transcription for ΔIP-H5 versus N315ΔIP and N315 rpoB(N967I) versus N315

Function and ORFaGeneProductbRatio of signal intensity (mutant to parent strain)
ΔIP-H5/N315ΔIPN315rpoB(N967I)/N315
Environmental information processing
    Autolysin-associated genes and regulators
        SA0252lrgAHolin-like protein; murein hydrolase regulator LrgA2.536.57
        SA0253lrgBAntiholin-like protein LrgB2.086.60
        SA2327cidCRegulatory protein for murein hydrolase activity0.440.26
        SA2328cidBRegulatory protein for murein hydrolase activity0.480.27
        SA2329cidARegulatory protein for murein hydrolase activity0.420.25
        SA1898sceD-likeSimilar to SceD protein, predicted to be a lytic transglycosylase2.213.26
    Other signal transduction and regulators
        SA2090rsrRepressor of sarR and agr genes0.300.38
        SA2091sarYStaphylococcal accessory regulator Y0.220.31
        SA2092Similar to transcription regulator AraC type regulator0.200.35
Metabolism
    Cell-wall biosynthesis and metabolism
        SA0124Similar to glycosyltransferase TuaA0.390.43
        SA0125Similar to exopolysaccharide G (EpsG)0.350.40
        SA0523Similar to poly(glycerol-phosphate) alpha-glucosyltransferase2.032.33
        SA1964fmtB (mrp)Cell surface protein; inactivation reduces MET resistance0.240.29
    Cell envelope biogenesis
        SA0126Similar to capsular polysaccharide synthesis protein 14H0.390.43
        SA0127Similar to capsular polysaccharide synthesis protein 14L0.470.50
    Carbon hydrate metabolism
    Nitrogen metabolism
    Energy metabolism
    Nucleotide transport and metabolism
        SA1047pyrFOrotidine-5-phosphate decarboxylase0.340.48
        SA1172Similar to GMP reductase0.320.47
    Sugar transport and metabolism
        SA0837Similar to 2-isopropylmalate synthase2.952.86
        SA1991lacG6-Phospho-beta-galactosidase0.290.23
        SA1992lacEPTS system, lactose-specific IIBC component0.290.25
        SA1993lacFPTS system, lactose-specific IIA component0.340.19
        SA1994lacDTagatose-1,6-diphosphate aldolase0.360.21
        SA1995lacCTagatose-6-phosphate kinase0.420.21
        SA1996lacBGalactose-6-phosphate isomerase LacB subunit0.490.25
        SA24862-Oxoglutarate/malate translocator homolog2.132.03
    Amino acid transport and metabolism
        SA0822argGArgininosuccinate synthase0.400.48
        SA0850Similar to oligopeptide ABC transporter oligopeptide-binding protein0.270.44
    Terpenoid metabolism
        SA1304Similar to component A of hexaprenyl diphosphate synthase2.242.19
    Secretion and other transport system
        SA0956Similar to Mn2+ transport protein0.200.32
        SA2203mdeASimilar to multidrug resistance protein2.362.14
        SA2442secA2Preprotein translocase secA homolog2.312.18
        SA2443asp3Accessory secretory protein Asp33.103.20
        SA2444asp2Accessory secretory protein Asp23.893.36
        SA2445asp1Accessory secretory protein Asp15.934.04
        SA2446secY2Similar to preprotein translocase secY5.514.01
Genetic information processing
    SA0706Similar to comF operon protein 32.343.16
    SA1899Similar to single-strand DNA binding protein2.412.56
Virulence factor
    SA0393ssl11Exotoxin 150.310.23
    SA0519sdrCSer-Asp-rich fibrinogen-binding, bone sialoprotein-binding protein2.593.48
    SA0520sdrDSer-Asp-rich fibrinogen-binding, bone sialoprotein-binding protein2.263.45
    SA0521sdrESer-Asp-rich fibrinogen-binding, bone sialoprotein-binding protein4.454.60
    SA1267ebhAExtracellular matrix-binding protein EbhA0.220.11
    SA1577sasCS. aureus surface protein C0.360.40
    SA2423clfBClumping factor B4.582.49
    SA2430aurZinc metalloproteinase aureolysin0.403.50
Hypothetical protein
    SA0262Hypothetical protein2.182.35
    SA0266Conserved hypothetical protein2.172.16
    SA0267Hypothetical protein2.102.21
    SA0268Hypothetical protein2.072.74
    SA0269Hypothetical protein2.002.30
    SA0271Conserved hypothetical protein2.413.90
    SA0275Conserved hypothetical protein2.182.16
    SA0331Conserved hypothetical protein0.310.33
    SA0332Conserved hypothetical protein0.230.35
    SA0333Conserved hypothetical protein0.260.35
    SA0394Hypothetical protein0.300.24
    SA0663Hypothetical protein0.320.48
    SA0737Hypothetical protein2.902.28
    SA0738Hypothetical protein2.552.37
    SA0739Conserved hypothetical protein2.865.11
    SA0741Conserved hypothetical protein3.934.14
    SA0742Hypothetical protein3.743.29
    SA0761Conserved hypothetical protein2.546.06
    SA0830Conserved hypothetical protein2.492.63
    SA1002Hypothetical protein2.403.25
    SA1049Hypothetical protein0.410.37
    SA1222Hypothetical protein2.042.49
    SA1268Hypothetical protein0.360.25
    SA1664Conserved hypothetical protein2.222.23
    SA1755Hypothetical protein (bacteriophage ϕN315)0.250.188
    SA2299Conserved hypothetical protein2.702.04
    SA2491Conserved hypothetical protein2.492.55
  • a Locus tags are based on S. aureus strain N315. ORF, open reading frame.

  • b MET, methicillin; PTS, phosphotransferase system.