Table 1

Resistance and cross-resistance characterization of the R0.8 bloodstream form cell line, compared to the parental wild-type T. brucei brucei strain Lister 427

CompoundAverage EC50 (μM)Resistance factorP valuea
Lister 427R0.8
PDE inhibitors
    CpdA0.08 ± 0.011.4 ± 0.217.20.004
    CpdB0.13 ± 0.031.28 ± 0.259.70.016
    Dipyridamole17.9 ± 2.79.2 ± 0.80.50.059
cAMP analogues
    Dibutyryl cAMP263 ± 131890 ± 3147.20.011
    8-Bromo-cAMP271 ± 81133 ± 1854.20.014
    8-(4-chlorophenylthio)-cAMP1.24 ± 0.40.25 ± 0.050.20.201
Known trypanocides
    Suramin0.0212 ± 0.00080.0156 ± 0.00050.70.001
    Diminazene0.022 ± 0.0070.011 ± 0.0010.50.133
    Pentamidine0.0016 ± 0.00040.0014 ± 0.00020.90.683
    Cymelarsen0.0038 ± 0.00040.0038 ± 0.00031.01.000
    Phenylarsine oxide0.00083 ± 0.000060.00088 ± 0.000111.10.783
    Nifurtimox2.01 ± 0.241.61 ± 0.080.80.246
  • a For comparison of R0.8 and Lister 427.