Table 2

Frequency of amino acid changes detected by 454 sequencing during selection with GSK5852 in genotype 1a and 1b replicon cells

Genotype 1aGenotype 1b
Substitution (codon change)Frequency (%)Substitution (codon change)Frequency (%)
5× EC5020× EC505× EC5020× EC50
I23V (ATC→GTC)8L127Q (CTG→CAG)3
V131A (GTA→GCA)3Q309K (CAG→AAG)11
N273S (AAC→AGC)4C316F (TGC→TTC)1
C316F (TGT→TTT)7C316Y (TGC→TAC)3990
C316Y (TGT→TAT)62S365L (TCA→TTA)126
S365L (TCA→TTA)2479S365T (TCA→ACA)3
S368F (TCC→TTC)217A395G (GCT→GGT)2
S368Y (TCC→TAC)23M414I (ATG→ATA)1
A377T (GCT→ACT)4M414L (ATG→TTG)1
L392F (CTC→TTC)2M414V (ATG→GTG)3
N411Y (AAC→TAC)1C445F (TGT→TTT)3862
N411S (AAC→AGC)1W574R (TGG→CGG)4
M414I (ATG→ATA)3I585T (ATC→ACC)1
M414V (ATG→GTG)1I585V (ATC→GTC)4