Table 3

Substitutions at positions 148 and 151 in the neuraminidase of a subset of A(H3N2) viruses passaged on MDCK and MDCK-SIAT1 cells using modified procedures

Virus IDSubstitution using modified procedure
MDCK cellsMDCK-SIAT1 cells
P1P2P3P4P5P1P2P3P4P5
148151148151148151148151148151148151148151148151148151148151
1aD/Gb
2T/IcIII
3D/N
4
5
6T/I/KI/KI/K
7T/ID/GT/II
8T/KT/K
9T/IT/II
10D/G
11D/GD/G
12NDdND
  • a No changes observed (wild type).

  • b D/G, mixture of aspartic acid and glycine at position 151.

  • c T/I, mixture of threonine and isoleucine at position 148.

  • d ND, not done; the attempt to reisolate the virus failed.