Table 4

IC50s of recombinant neuraminidases tested in the fluorescent NI assay

NA substitutionMean IC50 ± SDa [nM (foldb)]
ZanamivirOseltamivirPeramivirLaninamivir
E119V0.54 ± 0.13 (3)44.93 ± 3.76 (281)0.12 ± 0.02 (1)0.43 ± 0.08 (4)
T148I1.33 ± 0.16 (6)0.04 ± 0.00 (1)0.06 ± 0.00 (1)0.34 ± 0.02 (3)
E119V, T148I220.48 ± 14.00 (1,050)984.62 ± 76.60 (6,154)9.54 ± 1.67 (119)79.47 ± 10.30 (722)
None0.21 ± 0.030.16 ± 0.070.08 ± 0.010.11 ± 0.04
  • a Based on at least three independent experiments.

  • b Fold change based on comparison to the respective IC50 values of recNA E119 and T148 (wild type).