Table 4

Inhibition of macromolecule synthesis (EC50) in E. coli ATCC 25922a

CompoundMIC (μg/ml)EC50 (μg/ml)
ProteinRNADNAFatty acidCell wall
AN336548.3105366396474
Tetracycline0.50.2736>64>64>64
Rifampin8121282>128128
Ciprofloxacin0.0150.12>0.50.001>0.5>0.5
Triclosan≤0.0040.18>1>10.01>1
  • a MICs were determined in M9 minimal medium with 0.25% (wt/vol) yeast extract, and [14C]leucine, [14C]uridine, [14C]thymidine, [14C]acetic acid, and [14C]N-acetylglucosamine incorporation was used to measure protein, RNA, DNA synthesis, fatty acid synthesis, and cell wall synthesis, respectively.