Table 3

Apparent Km(Ki) and inhibition values for select N152 variantsa

β-LactamaseAmpicillin, Km(Ki) (nM)Tazobactam
IC50 (nM)KI (μM)kinact (s−1)
WT CMY-2240 ± 203,200 ± 40050 ± 200.050 ± 0.006
N152G120 ± 10900 ± 20060 ± 200.25 ± 0.04
N152S19 ± 2180 ± 3050 ± 200.30 ± 0.06
N152T47 ± 5330 ± 3030 ± 200.12 ± 0.03
  • a Values are reported as means ± standard deviations.