Table 1

Effects of wild-type peptides (hBD1 and hBD3) and analog peptides (1C, 3I, 3N, and 3NI) on A549 cell apoptosisa

Peptide% of apoptotic cells
24-h incubation48-h incubation72-h incubation
2.5 μM12.5 μM25.0 μM2.5 μM12.5 μM25.0 μM2.5 μM12.5 μM25.0 μM
Negative control1.21.21.21.41.41.42.32.32.3
hBD122.22.71.42.22.10.810.9
hBD33.62.94.51.52.23.60.823.7
1C2.21.72.33.22.52.91.61.61.2
3I1.81.82.02.82.93.01.822.5
3N2.21.63.44.23.34.81.32.11.5
3NI1.91.81.14.75.714.322.22.7
  • a Cells were incubated for 24, 48, or 72 h with all peptides at concentrations of 2.5, 12.5, and 25.0 μM. Data are expressed as a percentage of apoptotic cells after the subtraction of negative control values (untreated cells). No synthetic peptide significantly increased apoptosis versus the negative control. Experiments were performed in triplicate.