Table 2

Effects of wild-type peptides (hBD1 and hBD3) and analog peptides (1C, 3I, 3N, and 3NI) on CaCo-2 cell apoptosisa

Peptide% of apoptotic cells
24-h incubation48-h incubation72-h incubation
2.5 μM12.5 μM25.0 μM2.5 μM12.5 μM25.0 μM2.5 μM12.5 μM25.0 μM
Negative control4.24.24.26.66.66.66.36.36.3
hBD17.110.39.66.16.278.27.54.1
hBD312.612.820.88.7634.19.27.69.8
1C912.311.77.813.620.65.210.98
3I6.16.87.05.86.27.15.36.17.2
3N8.212.312.910.810.310.165.99.1
3NI6.69.214.37.56.214.27.36.36.2
  • a Cells were incubated for 24, 48, or 72 h with all peptides at concentrations of: 2.5, 12.5, and 25.0 μM. Data are expressed as a percentage of apoptotic cells after the subtraction of negative control values (untreated cells). No synthetic peptide significantly increased apoptosis versus the negative control. Experiments were performed in triplicate.