Table 3

Effects of wild-type peptides (hBD1 and hBD3) and analog peptides (1C, 3I, 3N, and3NI) on Capan-1 cell apoptosisa

Peptide% of apoptotic cells
24-h incubation48-h incubation72-h incubation
2.5 μM12.5 μM25.0 μM2.5 μM12.5 μM25.0 μM2.5 μM12.5 μM25.0 μM
Negative control15.215.215.210.210.210.219.619.619.6
hBD114.412.612.917.811.310.715.18.117.2
hBD3148.39.210.914.818.710.815.926.2
1C9.212.31416.116.216.2211822
3I7.07.38.19.210.311.415.216.817.6
3N8.47.416.714.712.511.212.825.222.3
3NI10.715.513.115.416.923.425.624.531.8
  • a Cells were incubated for 24, 48, and 72 h with all peptides at concentrations of 2.5, 12.5, and 25.0 μM. Data are expressed as a percentage of apoptotic cells after the subtraction of negative control values (untreated cells). No synthetic peptide significantly increased apoptosis versus the negative control. Experiments were performed in triplicate.