Table 1

Kinetic parameters for the carboxypeptidase activity of Pa sPBP5 at pH 9.5

Cell wall-based substrateMean Km (mM) ± SDMean kcat (s−1) ± SDMean kcat/Km (M−1 s−1) ± SD
Nα,Nϵ-Diacetyl-Lys-d-Ala-d-Ala8.7 ± 0.20.59 ± 0.0465 ± 5
Methyl NAM-l-Ala-γ-d-Glu-meso-DAP-d-Ala-d-Ala6.1 ± 1.40.31 ± 0.0555 ± 20