TABLE 2

BLAST analyses to validate the system under different stringent conditions using gene fragments of different percentages of lengtha

% length e-valueParameterValue(s) for gene fragment of length:
100%70%50%25%10%5%
XYXYXYXYXYXY
1E-100Sp (%)100096 (4)090 (10)074 (26)NoNoNoNoNo
Sn (%)0100010001000NoNoNoNoNo
1E-50Sp (%)100095 (5)089 (11)086 (14)081 (19)NoNoNo
Sn (%)01000100010001000NoNoNo
1E-25Sp (%)100094 (6)089 (11)086 (14)077 (23)066 (34)No
Sn (%)010001000100010001000No
1E-10Sp (%)100094 (6)089 (11)086 (14)081 (19)077 (23)0
Sn (%)010001000100010001000100
1E-5Sp (%)100094 (6)089 (11)084 (16)076 (24)074 (26)0
Sn (%)010001000100010001000100
  • a The table shows the specificity and sensitivity analysis of 100 randomly selected sequences (1.0E-100 to 1.0E-5). The e-values represent the different stringent conditions. X, randomly selected antibiotic resistance gene sequences from the database (see Fig. S8 in the supplemental material); Y, randomly selected gene sequences (no antibiotic resistance gene) from Escherichia coli, Acinetobacter baumannii, and Staphylococcus aureus genomes; Sp, specificity; Sn, sensitivity; No, no output. Values in parentheses show the numbers of specific hits at the group level.