TABLE 1

Bacterial strains and plasmids used in this study

Strain or plasmidPhenotypea or genotypeReference or origin
P. aeruginosa strains
    PAO1Wild-type reference strain PAO1 with resequenced genome24
    PAOW1PAO1 spontaneous mutant overexpressing MexXY24
    PAOW2PAO1 spontaneous mutant overexpressing MexXY with M59I substitution in response regulator ParR24
    CMZ091PAO1 ΔmexZ24
    CM096PAO1 ΔparRS24
    CM106CM096 cis complemented with parRS from PAOW2, Tcr24
    CM114PAO1 ΔmexXYThis study
    CM115PAO1 ΔoprMThis study
    CM116CM096 cis complemented with parRS from PAO1, TcrThis study
    CM141CM096 cis complemented with parRS from strain 2756, TcrThis study
    CM142CM096 cis complemented with parRS from strain 2794, TcrThis study
    CM143CM096 cis complemented with parRS from strain 2946, TcrThis study
    CM144CM096 cis complemented with parRS from strain 3136, TcrThis study
    CM145CM096 cis complemented with parRS from strain 3562, TcrThis study
    CM146CM096 cis complemented with parRS from strain 3726, TcrThis study
    CM147CM096 cis complemented with parRS from strain 3728, TcrThis study
E. coli strains
    CC118Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA154
    HB101supE44 hsdS20(rB mB) recA13 ara-14 pro A2 lacY1 galK2 rpsL20 xyl-5 mtl-1 leuB6 thi-1Invitrogen
Plasmids
    PCR2.1Cloning vector for PCR products, lacZΔColE1 f1 ori, Apr KmrInvitrogen
    pRK2013Helper plasmid, ColE1 ori, Tra+ Mob+ Kmr30
    mini-CTX1Self-proficient integration vector, tet Ω-FRT-attP-MCS ori int oriT, Tcr36
  • a Antibiotic selection markers: Tcr, tetracycline; Apr, ampicillin; Kmr, kanamycin. MCS, multiple cloning site.