TABLE 4

Genetic and phenotypic features of the eight agrW1 and five agrW2 mutant clinical strains

StrainGenotypeMutation in parRMutation(s) in parSMICa (μg/ml)Expression levelb
TOBAMKAPRCIPFEPCAZTICCSTmexYPA5471PA1797arnA
PAO1Wild type0.54160.25421611111
PAOW1agrW1216640.516216115.26.1−1.21.0
PAOW2agrW2M59I18640.58216216.0−1.692.9104.7
2405agrW14641280.58232139.56.01.2−5.0
2946agrW1R155Hc11632116264135.94.86.34.6
3136agrW1A82Tc2321280.58232118.23.5−1.2−1.4
3452agrW1432256328232116.210.13.3−2.2
3564agrW14322560.58116143.95.0−1.2−1.4
4192agrW118320.58232144.715.6−1.2−1.6
4592agrW1216644161664116.94.61.12.1
5013agrW111664116432151.37.44.0−1.4
2756agrW2A168Vd1163221646418−1.112.096.3
2794agrW2L99Pd416128232432228.71.340.717.6
3562agrW2L137Pd128e32640.5164>1,024e48.0−1.13.098.7
3726agrW2V152Ad216640.58216110.51.427.928.7
3728agrW2R83K, A138Td216641821624.31.24.611.0
  • a Abbreviations: TOB, tobramycin; AMK, amikacin; APR, apramycin; CIP, ciprofloxacin; FEP, cefepime; CAZ, ceftazidime; TIC, ticarcillin; CST, colistin.

  • b Mean values of two independent experiments, expressed as ratios versus PAO1 expression levels.

  • c Amino acid change with no effect on protein function, as demonstrated by gene cis complementation of mutant strain PAO1ΔparRS, CM096 (see Table S1 in the supplemental material).

  • d Amino acid change activating sensor ParS (see Table S1).

  • e Probable additional enzymatic mechanism of resistance.