TABLE 3

Quantitative assessment of the effects of SQ109 on the biosynthesis and export of sulfolipids, di- and polyacyltrehaloses, and phthiocerol dimycocerosates in M. tuberculosis

Whole-cell treatmenta% increase/decrease radioactivity compared to control forb:
TMMTDMDATPATAc2SLSL-IPDIMMBT
SQ109211−8873−68145−20−47−16
AU1235318−8470−4488−19−29−13
INH−72−82−18−43−9−25−63NDc
  • a All were at 10× the MIC.

  • b The amount of radioactivity incorporated by whole cells (including cell-associated and surface-exposed materials) in TMM, TDM, SL-I, the diacylated SL precursor (Ac2SL), DAT, PAT, PDIM, and (carboxy)mycobactins (MBT) of the M. tuberculosis SQ109-, AU1235-, and INH-treated cells was analyzed by TLC (Fig. 4 and data not shown) and semiquantified using a PhosphorImager. The results are expressed as percent increases or decreases of the values measured in the untreated control.

  • c ND, not determined.