TABLE 4

Cross-resistance of adamantyl urea and SQ109 spontaneous resistant mutants of M. tuberculosis H37Rv to AU1235, BM212, and SQ109

IsolateaMIC (μg/ml) forb:
WTcAU2AU3AU4AU5AU6DA5_1DA5_2DA8_1DA8_2
AU12350.2–0.4>1.6>1.6>1.6>1.6>1.6>1.61.6>1.61.6
BM2121.25105–1010551010105
SQ1091NDdNDNDNDND422–82–4
  • a The mutation for the isolates AU2, AU3, AU4, AU5, and AU6 is G253E, that for DA5_1 and DA5_2 is A700T, that for DA8_1 is L567P, and that for DA8_2 is Q40R. The adamantyl urea (isolates AU2, AU3, AU4, AU5, and AU6) and SQ109 (isolates DA5_1, DA5_2, DA8_1, and DA8_2) spontaneous resistant mutants of M. tuberculosis H37Rv were reported previously (9, 19).

  • b The MICs, defined as the concentration of inhibitors that inhibited 100% of the growth and metabolic activity of the various M. tuberculosis H37Rv isolates, were determined in 96-well microtiter plates using the colorimetric resazurin assay in 7H9-OADC-Tween 80 broth at 37°C.

  • c The wild-type (WT) strain was M. tuberculosis H37Rv TMC102.

  • d ND, not determined.