TABLE 1

Effects of AmB and allicin and their combination on promastigotes of Leishmania infantum determined by alamarBlue assaya

D1-D2 combination ratioAmB concn (μM)Allicin concn (μM)Growth Inhibition (%)bmDm (μM)r
0.01:50.00251.253.5 ± 0.59
0.0052.53.4 ± 0.98
0.0153.5 ± 0.45
0.02103.6 ± 2.84
0.042015.0 ± 1.32
0.084099.0 ± 0.03
0.168099.0 ± 0.022.65511.710.90
0.01:100.00252.53.1 ± 1.54
0.00553.3 ± 1.49
0.01104.0 ± 2.02
0.022052.0 ± 2.22
0.044097.0 ± 1.65
0.088099.0 ± 0.11
0.1616099.0 ± 0.121.99821.960.91
0.01:200.002551.42 ± 1.05
0.005102.09 ± 1.13
0.012037.5 ± 1.52
0.024089.0 ± 2.31
0.048099.0 ± 0.07
0.0816099.0 ± 0.04
0.1632099.0 ± 0.022.47826.300.94
  • a Drugs were combined at constant ratios (0.01:5, 0.01:10, and 0.01:20) and their dose-effect parameters were assessed by the Chou-Talalay method (17) using CalcuSyn software. Dm (median-effect dose) signifies the potency and it is the concentration which inhibits cell growth by 50%; m is the shape of the dose-effect curve, where m values of 1, >1, and <1 indicate hyperbolic, sigmoidal, and flat sigmoidal curves, respectively; r represents the linear correlation coefficient of the median-effect plot (conformity of the data to the mass action law principle). The m, Dm (μM), and r values for AmB (drug 1 [D1]) were 1.451, 0.09, and 0.86, respectively, and those for allicin (D2) were 2.066, 22.13, and 0.90, respectively.

  • b Results are expressed as the means ± standard deviations of three independent experiments.