TABLE 2

Peptidoglycan fragments from S. coelicolor A3(2) grown under various conditions

Lactoyl peptideb or parameterCalculated monoisotopic mass (Da)Relative abundance (%) upon growth in NMMP broth supplemented witha:
NothingVmVm + Gly
Monomers
    Di289.132300
    Tri461.2154014
    Tetra532.25163717
    Tetra (Gly)c518.230021
    Penta603.2983320
    Penta (Gly)d589.2703028
Dimers (cross-link)
    Tri-tri (3→3)1,018.4618181
    Tri-tetra (3→3)1,089.4939468
    Tri-tetra (Gly)c (3→3)1,075.480037
    Tetra-tri (4→3)1,089.492200
    Tetra-tetra (Gly)c (4→3)1,146.520023
    Tetra-tetra (4→3)1,160.53133623
    Tetra-penta (4→3)1,231.57700
    Tetra-penta (Gly)d (4→3)1,217.55008
Proportion (%) of 3→3 cross-links576446
  • a The growth medium was supplemented with vancomycin (Vm) at 10 μg/ml or glycine (Gly) at 20 mM as indicated. The relative abundances of monomers and dimers were calculated independently.

  • b The stem peptides linked to the d-lactoyl group consisted of dipeptides l-Ala–d-Glx (Di), tripeptides l-Ala–d-iGlx–l,l-A2pm(Gly) (Tri), tetrapeptides l-Ala–d-iGlx–l,l-A2pm(Gly)–d-Ala (Tetra), and pentapeptides l-Ala–d-iGlx–l,l-A2pm(Gly)–d-Ala–d-Ala (Penta). Lactoyl peptides containing d-iGlu and d-iGln were grouped (d-iGlx). Masses are provided for d-iGln-containing peptidoglycan fragments.

  • c Gly at the fourth position of tetrapeptide stems in monomers and in the acceptor position of dimers.

  • d Gly at the fifth position of pentapeptide stems in monomers and in the acceptor position of dimers.