TABLE 1

Analysis of promoter transcription fusion constructs

StrainC23O activity (Uc/OD450)a
KJ(pSmeOxylE)18 ± 1.7
KJΔRo(pSmeOxylE)89 ± 9.2
KJ(pSmeRoxylE)20 ± 3.0
KJΔRo(pSmeRoxylE)54 ± 4.5
KJ(pPCMxylE)119 ± 17
KJΔRo(pPCMxylE)115 ± 14
KJ(pTolCxylE)5 ± 1
KJΔRo(pTolCxylE)8 ± 1
  • a One unit of catechol 2,3-dioxygenase (Uc) is defined as 1 nmol of catechol hydrolyzed per min. Results are expressed as the means ± standard deviations of three independent determinations. OD450, optical density at 450 nm.