TABLE 5

Pseudomonas aeruginosa mutant isolate MICs

StrainaMutation(s)MIC (μg/ml)b
NBTI 5463LVXCIPFEPTETMEM
PAO1 parent0.50.50.12184
AZ301nfxB 4-bp deletion at bp 490–4938414320.5
AZ302nfxB stop codon at bp 1008418320.5
AZ303nfxB multiple mutations from bp 341 onwards (including stop codon)410.54160.25
AZ304nfxB deletion at bp 42–2678414320.5
AZ-LV-1GyrA T83I<0.06341184
AZ-LV-2GyrA T83I<0.063821168
AZ-LV-3GyrB E468D0.2542284
  • a The AZ-LV mutants were three independent mutants selected on 4× MIC of levofloxacin. Several additional levofloxacin-resistant strains were also characterized; all had gyrase mutations.

  • b Abbreviations: LVX, levofloxacin; CIP, ciprofloxacin; FEP, cefepime; TET, tetracycline; MEM, meropenem.