Summary of activity parameters for tyrocidines against C. albicansa
| Compound | MIC (MICmax)b | MIC50c | BIC (BICmax)d | BIC50e | BEC50f | % biofilm eradication at 200 μM |
|---|---|---|---|---|---|---|
| Trc mix | 6.25* (5.99 ± 0.26)* | 3.72 ± 0.25* | 12.5* (11.3 ± 0.67)* | 8.30 ± 0.43* | 107 ± 20* | 81 ± 3.3* |
| TrcA | 6.25 (5.21 ± 0.44) | 3.19 ± 0.31 | 12.5 (8.01 ± 1.77) | 5.68 ± 0.71 | 145 ± 23 | 64 ± 4.8 |
| TrcB | 6.25 (6.25 ± 0.00) | 4.31 ± 0.03 | 12.5 (10.6 ± 0.32) | 8.29 ± 0.11 | 164 ± 18 | 55 ± 7.3 |
| TrcC | 6.25 (7.81 ± 0.81) | 4.65 ± 0.05 | 25.0 (24.3 ± 1.44) | 17.4 ± 0.20 | 133 ± 6.3 | 74 ± 3.3 |
| PhcA | 12.5 (12.5 ± 0.00) | 7.93 ± 0.48 | 25.0 (25.6 ± 2.54) | 15.1 ± 2.08 | >200 | 41 ± 3.5 |
| TpcC | 6.25 (5.99 ± 0.26) | 4.16 ± 0.42 | 25.0 (18.4 ± 3.33) | 15.5 ± 0.91 | >200 | 28 ± 5.4 |
| GS | 6.25 (6.77 ± 0.52) | 4.38 ± 0.03 | 12.5 (8.55 ± 0.57) | 5.74 ± 0.29 | 40.9 ± 6.2 | 100 ± 0.04 |
| CAS | 0.06 (0.06 ± 0.00) | 0.04 ± 0.01 | 0.16 (0.10 ± 0.01) | 0.06 ± 0.00 | 0.35 ± 0.03 | NDg |
| AMB | 0.80 (0.79 ± 0.03) | 0.26 ± 0.01 | 1.25 (0.82 ± 0.13) | 0.35 ± 0.05 | 0.56 ± 0.02 | ND |
↵a Each value represents the mean of ≥3 biological repeats, with 2 to 5 technical repeats per assay. The values are given in μM or, where indicated with an asterisk, μg/ml. Values in parentheses are means ± SEM.
↵b The MIC value is the lowest concentration in assay that resulted in visible C. albicans planktonic cell death compared to the growth control. MICmax values were calculated from dose-response curves, according to Rautenbach et al. (46), for statistical analyses.
↵c Lowest concentration in assay that prevents 50% planktonic cell growth, as derived from dose-response curves for statistical analyses (46).
↵d The BIC value is the lowest concentration in assay that prevented visible biofilm formation compared to the growth control. BICmax values were calculated from dose-response curves, according to Rautenbach et al. (46), for statistical analyses.
↵e Lowest concentration in assay that prevents biofilm formation by 50%, as derived from dose-response curves for statistical analyses (46).
↵f Lowest concentration in assay that eradicates 50% of the biofilm cells, as derived from dose-response curves for statistical analyses (46).
↵g ND, not determined.