TABLE 2

Summary of activity parameters for tyrocidines against C. albicansa

CompoundMIC (MICmax)bMIC50cBIC (BICmax)dBIC50eBEC50f% biofilm eradication at 200 μM
Trc mix6.25* (5.99 ± 0.26)*3.72 ± 0.25*12.5* (11.3 ± 0.67)*8.30 ± 0.43*107 ± 20*81 ± 3.3*
TrcA6.25 (5.21 ± 0.44)3.19 ± 0.3112.5 (8.01 ± 1.77)5.68 ± 0.71145 ± 2364 ± 4.8
TrcB6.25 (6.25 ± 0.00)4.31 ± 0.0312.5 (10.6 ± 0.32)8.29 ± 0.11164 ± 1855 ± 7.3
TrcC6.25 (7.81 ± 0.81)4.65 ± 0.0525.0 (24.3 ± 1.44)17.4 ± 0.20133 ± 6.374 ± 3.3
PhcA12.5 (12.5 ± 0.00)7.93 ± 0.4825.0 (25.6 ± 2.54)15.1 ± 2.08>20041 ± 3.5
TpcC6.25 (5.99 ± 0.26)4.16 ± 0.4225.0 (18.4 ± 3.33)15.5 ± 0.91>20028 ± 5.4
GS6.25 (6.77 ± 0.52)4.38 ± 0.0312.5 (8.55 ± 0.57)5.74 ± 0.2940.9 ± 6.2100 ± 0.04
CAS0.06 (0.06 ± 0.00)0.04 ± 0.010.16 (0.10 ± 0.01)0.06 ± 0.000.35 ± 0.03NDg
AMB0.80 (0.79 ± 0.03)0.26 ± 0.011.25 (0.82 ± 0.13)0.35 ± 0.050.56 ± 0.02ND
  • a Each value represents the mean of ≥3 biological repeats, with 2 to 5 technical repeats per assay. The values are given in μM or, where indicated with an asterisk, μg/ml. Values in parentheses are means ± SEM.

  • b The MIC value is the lowest concentration in assay that resulted in visible C. albicans planktonic cell death compared to the growth control. MICmax values were calculated from dose-response curves, according to Rautenbach et al. (46), for statistical analyses.

  • c Lowest concentration in assay that prevents 50% planktonic cell growth, as derived from dose-response curves for statistical analyses (46).

  • d The BIC value is the lowest concentration in assay that prevented visible biofilm formation compared to the growth control. BICmax values were calculated from dose-response curves, according to Rautenbach et al. (46), for statistical analyses.

  • e Lowest concentration in assay that prevents biofilm formation by 50%, as derived from dose-response curves for statistical analyses (46).

  • f Lowest concentration in assay that eradicates 50% of the biofilm cells, as derived from dose-response curves for statistical analyses (46).

  • g ND, not determined.