Summary of activity parameters for AMB and CAS in combination with TrcA, TrcB, and TrcC against 24-h-old C. albicans biofilmsa

Tyrocidine concn (μM)Plus TrcAPlus TrcBPlus TrcC
  • a Each value represents the mean of ≥3 biological repeats, with 2 to 5 technical repeats per assay ± standard error of the mean (SEM).

  • b The BEC50 value, derived from a full dose-response curve, represents the concentration AMB or CAS at which 50% eradication was achieved in the presence of a constant tyrocidine concentration. The AMB and CAS dose-response concentration ranges were 0.04 to 5.0 μM and 0.02 to 2.5 μM, respectively.

  • c The fractional inhibitory concentration index (FICI) was calculated using the BEC50 obtained for combinatorial treatment and the BEC50 values for the individual compounds (Table 2).