TABLE 1

Characteristics of strains used in this study

Bacterial species and strainsPulsed-field typePhenotypeaIsolate sourceSite of isolation
Pseudomonas aeruginosa
    SAMMC-6041MDRWound cultureTissue deep
    SAMMC-0152MDRBloodBlood
    SAMMC-8862MDRWound cultureTissue deep
    SAMMC-41818MDRWoundTissue deep
    SAMMC-18918MDRBloodBlood
    PAO1 (ATCC 15692)bUnknownUnknownWoundUnknown
Staphylococcus aureus
    SAMMC-700USA 100MRSAWound cultureTissue deep
    SAMMC-641USA 200MRSAWound cultureTissue deep
    SAMMC-474USA 800MRSAWound cultureTissue deep
    SAMMC-446USA 300MRSAWound cultureTissue deep
    SAMMC-240USA 300MRSAWound cultureTissue deep
    UAMS-1 (ATCC 43290)cUSA 200MSSAWound cultureBone
  • a A multidrug-resistant (MDR) organism was defined as an organism resistant to antimicrobials in ≥3 classes of antimicrobial agents (penicillins/cephalosporins, carbapenems, aminoglycosides, and quinolones), not including tetracyclines or colistin. MRSA, methicillin-resistant Staphylococcus aureus.

  • b Pseudomonas aeruginosa strain PAO1 is a well-characterized and commonly used wound isolate (34, 35).

  • c Staphylococcus aureus strain UAMS-1 is a methicillin-susceptible and well-characterized osteomyelitis isolate of S. aureus (32, 33).