TABLE 3

FKS sequencing resultsa

Anidula MIC (μg/ml)Caspo MIC (μg/ml)Mica MIC (μg/ml)Flucon MIC (μg/ml)Gene and hot spot region(s)
FKS1 HS1FKS2 HS1
0.1250.250.008128WTWT
0.0150.250.00832WTWT
0.030.50.0084WTWT
0.0160.250.0084WTWT
0.1250.250.0161WTWT
0.0160.50.01664WTWT
0.030.250.0168WTWT
0.1250.250.0168WTWT
0.030.250.034WTWT
0.250.1250.03128WTWT
0.1250.250.03128WTWT
0.1250.250.034WTWT
0.1250.250.034WTWT
0.060.250.064WTWT
0.1250.250.0664WTWT
0.1250.50.0664WTWT
0.250.1250.064WTWT
0.250.1250.064F L I L S L R Y PWT
0.50.250.1252WTF L I L F L R D P
0.030.250.125128WTWT
0.50.50.12564F L I L P L R D PWT
0.50.1250.12564F L I L P L R D PWT
0.1250.50.12516WTWT
0.1250.1250.1258WTWT
0.060.060.1254WTWT
0.50.50.1254WTWT
220.250.25WTF L I L S L R D H
10.50.25256WTY L I L S L R D P
120.25256WTY L I L S L R D P
0.060.1250.2564F L I L S L G D PWT
220.2564WTF L I L F L R D P
110.2532WTS L I L S L R D P
0.060.1250.2532F L I L S L G D PWT
110.2516F L I L S L R V PWT
110.258WTY L I L S L R D P
0.060.1250.258WTWT
0.060.060.258WTWT
0.030.060.254I634VWT
0.030.030.254WTWT
120.254WTY L I L S L R D P
0.510.254WTY L I L S L R D P
220.52WTF L I L P L R D P
0.50.250.51F L I L S L G D PF L I L S L R V P
0.250.250.5128F L I L S L G D PWT
110.5128WTdelF658
0.030.060.58WTWT
0.1250.1250.54F L I L P L R D PF L I L S L R V P
0.030.030.54WTWT
10.514F L I L P L R D PWT
0.250.512F L I L S L G D PWT
281256F L I L P L R D PWT
24164F L I L P L R D PF L I L S L R V P
216164WTF L I L P L R D P
14164WTF L I L P L R D P
2214WTWT
2424WTF L I L P L R D P
0.5124WTF L I L P L R D P
482128WTF L I L P L R D P
212128WTF L I L P L R D P
2>162128WTF L I L P L R D P
2>16264WTF L I L P L R D P
4>16216WTF L I L P L R D P
4>16216F L I L P L R D PWT
41628WTF L I L P L R D P
4>1628WTdelF658
11628WTF L I L P L R D P
4>1628WTF L I L P L R D P
21624WTF L I L P L R D P
4>1644WTF L I L P L R D P
2844WTF L I L P L R D P
0.5164256WTF L I L P L R D P
2164128WTF L I L P L R D P
4>16464WTF L I L P L R D P
216432WTF L I L P L R D P
416432WTF L I L P L R D P
  • a Among the 77 isolates with at least one echinocandin MIC in the intermediate or resistant range, FKS sequencing identified 47 with FKS mutations. Three of the isolates had a mutation that was one amino acid outside the recognized hot spot region. All of the mutations were in FKS1 hot spot 1 or FKS2 hot spot 1. This includes 47/55 (85%) isolates that showed resistance to at least one echinocandin. Anidula, anidulafungin; Caspo, caspofungin; Mica, micafungin; Flucon, fluconazole; WT, wild type. Boldface indicates the amino acid that has been changed in the hot spot.