TABLE 1

Results of retesting isolated strains with divergent results from PZA MIC measurements using the Bactec MGIT 960 system, pncA gene sequencing, PZase analysis, and VNTR analysis

Original strain no.Results for original strainIsolated strain no.Results for isolated strain
MGIT PZA MIC (μg/ml)pncA mutationPZase analysisMGIT PZA MIC (μg/ml)pncA mutationPZase analysisVNTRa
4>1,600G insertion at 392Positivei≤100NonePositiveConsistent in all loci
ii>1,600G insertion at 392Negative
6400NonePositivei<100NonePositive
13>1600NonePositivei≤100NonePositiveConsistent in all loci
ii>1,600NonePositive
17400NonePositivei≤100NonePositiveQUB11b:7b
ii400NonePositiveQUB11b:6b
iii800NonePositiveQUB11b:7b
25800NonePositivei≤100NonePositiveConsistent in all loci
ii400NonePositive
iii>1,600T→G at 456, V139GNegative
50200NonePositivei≤100NonePositiveConsistent in all loci
ii400NonePositive
79200NonePositivei≤100NonePositiveConsistent in all loci
ii400NonePositive
  • a Compared to results for the isolated strain among the Supply et al. (14) 15-locus VNTR.

  • b Recognized a difference in the copy number at QUB11b.