TABLE 1

E. coli gyrBA fusion complements temperature-sensitive growth of E. coli KNK453 [gyrA(Ts)] and N4177 [gyrB(Ts)]

Temperaturea (°C)KNK453 [gyrA(Ts)]b,cN4177 [gyrB(Ts)]c
pET28apET28a-gyrBpET28a -gyrApET28a -gyrBApET28apET28a-gyrApET28a-gyrBpET28a- gyrBA
30++++++++
42++++
  • a A temperature of 30°C is the permissive temperature, and 42°C is the restrictive temperature for E. coli strains KNK453 and N4177.

  • b For KNK453, 0.5 mM IPTG was added in LB plates to induced gyrA and gyrBA.

  • c +, Robust growth; −, no growth.