TABLE 2

E. coli gyrBA fusion reduces quinolone MICs for E. coli J53 (gyrA S83L)

Host cellsPlasmidMIC (μg/ml) for:
CiprofloxacinNorfloxacinNalidixic acid
E. coli J53 (gyrA S83L)None0.514,096
pET28a0.514,096
pET28-gyrB0.514,096
pET28-gyrA0.250.5512
pET28-gyrBA0.1250.25256
Wild-type E. coli J53None0.0160.068
pET28a0.0160.068
pET28-gyrB0.0160.068
pET28-gyrA0.0160.068
pET28-gyrBA0.0160.068