TABLE 4

qnrB protects gyrBA from ciprofloxacin inhibition in N4177 and KNK453

Host cellsPlasmidMIC (μg/ml) for:
30°C42°C
pTrcHisApTrcHisA-qnrB1pTrcHisApTrcHisA-qnrB1
E. coli N4177None0.0080.25
pET28a0.0080.25
pET28a-gyrA0.0080.25
pET28a-gyrB0.0080.250.0160.25
pET28a-gyrBA0.0080.250.0160.25
E. coli KNK453aNone0.061
pET28a0.061
pET28a-gyrB0.061
pET28a-gyrA0.0610.061
pET28a-gyrBA0.0610.061
  • a IPTG 0.5 mM was added to induce GyrA and GyrBA expression in E. coli KNK453.