Biophysical characteristics of the purified enzymes

Enzymea% solubleYield (mg/liter)Mol wt from ESI-MS (theoretical mol wt)Tm (°C)No. of Zn(II) ions/enzyme moleculeb
IMP-1-V67A257.425,088 (25,085)711.8 ± 0.1
IMP-1 (V67)805.125,112 (25,113)701.8 ± 0.1
IMP-1-V67I2515.125,129 (25,127)721.6 ± 0.1
IMP-10 (F67)505.625,160 (25,161)672.3 ± 0.2
  • a The enzymes are presented by increasing size of residue 67, with the smallest being alanine (IMP-1-V67A) and the largest being phenylalanine (IMP-10). The amino acid identities are shown in bold. Data for IMP-1 are from reference 33.

  • b Data are average values for three measurements ± standard deviations.