TABLE 1

Enterococcus faecium strains used in this study

E. faecium strainRelevant characteristic(s)aDAP MIC (μg/ml)Reference(s) or source
R446DAP- and VAN-resistant clinical isolate harboring eight changes in predicted proteins compared to its parental strain, including an S333L substitution in YycG, along with other changes in Cls, Cfa, RrmA, SulP, XpaC, and PTS-EIIA and in a protein harboring an HD domain166, 9, 20,21
R446FFusidic acid-resistant derivative of R44616This study
R446F ΔliaRDerivative of R446F harboring a nonpolar deletion of liaR0.25This study
R497DAP-resistant isolate harboring W73C and T120A substitutions in LiaR and LiaS, respectively, and an additional insertion of MPL at position 110 in Cls249, 21
R497FFusidic acid-resistant derivative of R49724This study
R497F ΔliaRDerivative of R497F harboring a nonpolar deletion of liaR0.094This study
R497F ΔliaR::liaRComplementation of liaR in cis (native chromosomal location)24This study
HOU503DAP-tolerant and VAN-resistant clinical isolate harboring W73C and T120A substitutions in LiaR and LiaS, respectively321
HOU503FFusidic acid-resistant derivative of HOU5033This study
HOU503F ΔliaRDerivative of HOU503F harboring a nonpolar deletion of liaR0.064This study
HOU503 ΔliaR::liaRComplementation of liaR in cis (native chromosomal location)3This study
HOU515DAP-tolerant clinical isolate harboring an A414T substitution in YycG and no LiaFSR substitutions321
HOU515FFusidic acid-resistant derivative of HOU5153This study
HOU515F ΔliaRDerivative of HOU515F harboring a nonpolar deletion of liaR0.047This study
  • a VAN, vancomycin; DAP, daptomycin; YycG, putative histidine kinase of the essential two-component regulatory system YycFG; PTS, phosphotransferase system; Cls, cardiolipin synthase.