TABLE 1

RT-PCR gene expression analysis with strains of B. cenocepacia J2315

StrainExpression relative to that of the WTa
ampCbpenBpenR (ampRc)
WT and mutants
    WT111
    M-Dup1d8,724.7 ± 2,861.7750.3 ± 413.21.1 ± 0.2
    M-Del68,110.0 ± 2,846.1493.8 ± 82.80.8 ± 0.2
    M-In15,494.6 ± 1,780.3407.2 ± 24.80.7 ± 0.2
    M-Sub52,489.1 ± 640.4243.5 ± 4.70.5 ± 0.1
    M-Sub81,999.0 ± 666.6241.4 ± 38.70.4 ± 0.2
    M-Sub141,508.0 ± 441.9234.1 ± 18.70.4 ± 0.1
    M-Ext2600.4 ± 131.7135.5 ± 20.50.4 ± 0.0
WT with inductione using:
    AMX62.2 ± 8.940.4 ± 2.10.6 ± 0.1
    CAZ12.4 ± 1.014.3 ± 1.60.8 ± 0.1
    CTXM23.4 ± 6.138.6 ± 3.60.4 ± 0.1
    MER409.1 ± 76.4139.5 ± 6.91.1 ± 0.1
  • a Wild-type strain.

  • b Target gene for the expression analysis.

  • c penR in B. cenocepacia J2315 is an ampR homolog.

  • d ampD mutation in the strain.

  • e Abbreviations for antibiotics used in the induction: AMX, amoxicillin; CAZ, ceftazidime; CTXM, cefotaxime; MER, meropenem.