TABLE 6

MICs of antibioticsa for reconstructed Amdr mutants and E. coli MG1655

StrainGenotypeMIC (mg/liter) of:
AmdMemCtxAmpCipNitFofTmpSxtChlNalTetEry
DA5438bWild type0.1250.0230.06420.01620.50.1250.04763116
DA28432ppa (D11A)960.0320.0470.750.01630.190.940.03240.50.75198
DA28434aspS (P191S)240.0470.04720.01630.0940.1250.032630.564
DA28436ubiE (delc bp 346–349 → FSd)120.0230.03210.01620.0940.750.0641.530.564
DA28438spoT (S305P)320.0320.0641.50.0160.250.190.1250.023320.09432
DA28439cysB KOe320.0640.04720.02310.50.380.064430.75>256
DA29703thrS (E519G)320.0320.06420.023410.250.047630.7524
DA29705mrdA (D389G)160.0230.0230.750.0161.50.380.940.016630.524
DA29707ubiX (del bp 246 → FS)320.0470.03210.02310.0940.250.04733196
DA29710rplL (promoter −10)480.0320.0470.50.02320.1250.1250.032430.7524
DA29712rpoB (H447L)480.0470.06420.02340.0640.0940.047430.7516
DA29715trxB (G37D)0.0640.0230.04720.016610.190.04743124
DA29717mrdA (D389G) trxB (G37D)640.0160.0230.750.02320.190.380.032660.548
DA30120gltX (D180G)240.0470.03210.0160.750.250.0940.032420.596
DA30122ispA (IS1 insertion)160.0320.02310.01620.0940.1250.032120.3896
  • a Mem, meropenem; Ctx, cefotaxime; Cip, ciprofloxacin; Nit, nitrofurantoin; Fof, fosfomycin; Tmp, trimethoprim; Sxt, trimethoprim-sulfamethoxazole; Nal, nalidixic acid; Ery, erythromycin.

  • b E. coli MG1655.

  • c del, deletion.

  • d FS, frame shift.

  • e KO, knockout.