TABLE 1

Characteristics of the bacterial isolates used

IsolateSourceMIC (μg/ml)Resistance mechanism(s)
MinocylineMinocyline + PAβNaPolymyxin B
AB BAA 747Laboratory0.250.1251Reference adeB expression
AB 7283Clinical0.50.1251Moderate overexpressionb of adeB
AB 1261Clinical10.1252Moderate overexpression of adeB
AB 7416Clinical1621tetB, moderate overexpression of adeB
  • a PAβN, Phe-Arg b-naphtylamide (100 μg/ml).

  • b Moderate overexpression, >10× the transcription level by qRT-PCR, compared to that in a wild-type isolate susceptible to minocycline.