TABLE 1

Strains and plasmids used in this study

Strain or plasmidaDescriptionbReference
Strains
    OG1RFParent strain; Rifr Fusr69
    OG1SSp(pCF10)Conjugative donor strain; Smr Spr Tetr70
    JD100OG1RF ΔfsrAThis work
    JD102OG1RF ΔepaOXThis work
    JD106OG1RF ΔepaIThis work
    TX5264OG1RF ΔgelE56
Plasmids
    pMSP3535Nisin-inducible expression vector; Emr29
    pCI372spcShuttle vector; SprThis work
    pJLD3fsrA complementation vector; pCI372spc derivedThis work
    pJLD4epaOX complementation vector; pCI372spc derivedThis work
    pMSP3614gelE complementation vector; pMSP3535 derived51
    pCQP1epaI complementation vector; pMSP3535 derivedThis work
    pCJK205P23::lacZ constitutive reporter vector; pMSP3535 derived47
  • a OG1SSp(pCF10) required 250 μg/ml spectinomycin; E. faecalis strains harboring pCI372spc derivatives required 1,000 μg/ml spectinomycin. E. faecalis isolates harboring pMSP3535 derivatives were cultured with 25 ng/ml nisin except when used in MIC assays.

  • b Rifr, rifampin resistant; Fusr, fusidic acid resistant; Smr, streptomycin resistant; Spr, spectinomycin resistant; Tetr, tetracycline resistant; Emr, erythromycin resistant.