TABLE 3

E. faecalis transposon mutants identified in this study

MutantOG1RF annotationaDescriptionbCoordinate 5′ to 3′ position (%)c% biofilm
Without antibioticWith antibioticd
13 B10OG1RF_11529 (fsrA)Quorum-sensing response regulator1592156 (3)77 ± 1639 ± 20 (DAP), 51 ± 7 (Lz), 87 ± 4 (Gm)
20 F7OG1RF_11527 (fsrC)Quorum-sensing histidine kinase1590604 (1)119 ± 2259 ± 14 (DAP)
100 E5OG1RF_11526 (gelE)Gelatinase1588069 (64)113 ± 920 ± 1 (DAP)
124 E8OG1RF_11730 (epaI)Group 2 glycosyltransferase1813805 (84)84 ± 2223 ± 4 (DAP)
6 A7OG1RF_11715 (epaOX)Glycosyltransferase1794475 (82)76 ± 753 ± 5 (DAP), 71 ± 9 (Gm)
  • a OG1RF annotation designated by the NCBI (INSDC accession no. CP002621.1) for the transposon-disrupted gene.

  • b Open reading frame description assigned by the NCBI.

  • c Coordinate positions were mapped to the OG1RF reference genome by the University of Minnesota Genomic Center. Percentages indicate the position of the transposon insertion from the 5′ end of the respective gene.

  • d DAP, daptomycin; Lz, linezolid; Gm, gentamicin.