TABLE 1

Chemical-genetic profiling of carvacrola

Gene nameDescription of productZ-score
IRE1Serine-threonine kinase and endoribonuclease; mediates the unfolded protein response by regulating Hac1p synthesis through HAC1 mRNA splicing3.03
HAC1Transcription factor; regulates the unfolded protein response2.83
TRP3Indole-3-glycerol-phosphate synthase1.92
ARO2Chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate to form chorismate, which is a precursor to aromatic amino acids1.87
ARO1Pentafunctional arom protein; catalyzes steps 2 through 6 in the biosynthesis of chorismate1.86
BCK1MAPKKK acting in the protein kinase C signaling pathway1.79
PHO84Hig high-affinity inorganic phosphate transporter1.63
YPT6Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi complex1.62
TRP2Anthranilate synthase; catalyzes the initial step of tryptophan biosynthesis1.56
TLG2Syntaxin-like t-SNARE; mediates fusion of endosome-derived vesicles with the late Golgi complex1.48
CWH41ER type II integral membrane N-glycoprotein involved in assembly of cell wall β-1,6-glucan and asparagine-linked protein glycosylation1.47
GTR2Putative GTP binding protein; negatively regulates Ran/Tc4 GTPase cycle1.36
SLT2Serine/threonine MAP kinase involved in regulating maintenance of cell wall integrity, cell cycle progression, and nuclear mRNA retention1.32
TRP4Anthranilate phosphoribosyl transferase; transferase of the tryptophan biosynthetic pathway1.30
MDM31Mitochondrial protein that may have a role in phospholipid metabolism1.22
SMI1Protein involved in the regulation of cell wall synthesis1.14
SWI6Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; required for the unfolded protein response1.13
COG6Component of the conserved oligomeric Golgi complex; functions in protein trafficking to mediate fusion of transport vesicles to Golgi complex1.12
YPS7Putative GPI-anchored aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum1.11
RPS8AProtein component of the small (40S) ribosomal subunit1.01
TRS85Component of transport protein particle complex III; regulates endosome-Golgi complex traffic and required for membrane expansion1.01
TRP1Phosphoribosylanthranilate isomerase; catalyzes the third step in tryptophan biosynthesis1.00
  • a Identification by HCGP assay of gene deletion mutants that confer sensitivity to carvacrol. Fitness defect scores were calculated based on bar code microarray hybridization, and the top 22 sensitive deletion strains sorted by Z-score are shown.