TABLE 2

Effect of cprA2 deletion in the PAK strain on pmrAB12-induced cationic antimicrobial peptide resistance

Strain/genotypePMBaProtegrin-1C18G
EC50 (mg/liter)FIREC50 (mg/liter)FIREC50 (mg/liter)FIR
PAK WT<0.12510.125121
PAK ΔpmrAB pJN105<0.12510.125121
PAK ΔpmrAB pJN105D::pmrAB1232>2561296256128
PAK ΔcprA2 ΔpmrAB pJN105D::pmrAB122>162166432
  • a EC50, effective concentration that kills 50% of CFU; FIR, fold increase in resistance to the indicated antimicrobial peptide of each strain with respect to PAK WT (ratio of EC50s).