TABLE 3

Relative abundance of cprA and arnC mRNA transcripts in phoQ deletion and pmrAB12 or cprRS21 expression backgrounds, determined by RT-qPCR analysis (n = 3 biological replicates)

GenotypeRelative mRNA abundance ± SDa
cprAarnC
PAK ΔphoQ1 ± 0.434 ± 17
PAK ΔpmrAB pJN105D::pmrAB1294 ± 2621 ± 7
PAK ΔcprRS pJN105D::cpRS21165 ± 3734 ± 18
PAK ΔcprA2 ΔcprRS pJN105D::cpRS21NA30 ± 9
PAK ΔarnC ΔcprRS pJN105D::cpRS21172 ± 70NA
PAK ΔphoQ ΔcprRS pJN105D::cprRS21236 ± 16251 ± 40
  • a Normalized to mRNA abundance in the PAK WT strain. NA, not applicable.