TABLE 2

Phenotypic and genotypic traits of metallo-β-lactamase-producing soil strains and closely related reference strains

Soil strainMeropenem MIC (μg/ml)Reference strain with closest 16S rRNA gene identity to soil straina
Name (accession no.)16S rRNA identity (%)CarbaNP test resultMeropenem MIC (μg/ml)
P. roseus strain SI-338P. roseus CL-GP80T (DQ112353)99.78NDND
P. kyungheensis THGT17 (JN196132)98.40NDND
P. borealis DSM19626 (EU030687)98.13NDND
P. agri PB92T (EF660751)98.00Negative8
P. heparinus CCUG 12810 (AJ438172)96.29Negative0.125
P. africanus CCUG 39353 (AJ438171)95.40Negative0.5
P. boryungensis CCUG 60024 (HM640986)95.32Negative0.125
P. saltans CCUG 39354 (AJ438173)91.31Negative0.064
P. kyungheensis strain Stok-3>32P. kyungheensis THGT17 (JN196132)98.16NDND
P. agri PB92T (EF660751)97.92Negative8
P. roseus CL-GP80T (DQ112353)97.46NDND
P. borealis DSM19626 (EU030687)97.31NDND
P. heparinus CCUG 12810 (AJ438172)95.69Negative0.125
P. africanus CCUG 39353 (AJ438171)95.62Negative0.5
P. boryungensis CCUG 60024 (HM640986)95.17Negative0.125
P. saltans CCUG 39354 (AJ438173)90.71Negative0.064
P. borealis strain ALS-14>32P. borealis DSM19626T (EU030687)98.94
P. agri PB92T (EF660751)98.52Negative8
P. kyungheensis THGT17T (JN196132)97.85
P. roseus CL-GP80T (DQ112353)97.46
P. heparinus CCUG 12810 (AJ438172)95.77Negative0.125
P. africanus CCUG 39353 (AJ438171)95.47Negative0.5
P. boryungensis CCUG 60024 (HM640986)94.88Negative0.125
P. saltans CCUG 39354 (AJ438173)90.64Negative0.064
E. tenax strain Stok-24E. tenax DSM 16811 (AF493696)98.24Positive3
E. lactis DSM19921 (EF204460)98.00Positive3
C. piscium strain Stok-14C. piscium CCUG 51923 (AM040439)98.58Positive3
C. balustinum CCUG 13228 (M58771)98.58Positive1.5
C. scophthalmum CCUG 33454 (AJ271009)98.07Positive3
Sphingomonas sp. strain ALS-13>32S. hankookensis CCUG 57509 (FJ194436)95.71Positive>32
S. aquatilis CCUG 48825 (AF131295)94.93Positive8
S. adhaesiva CCUG 27819 (D16146)94.57PositiveND
S. paucimobilis CCUG 6518 (D16144)94.21Negative0.047
S. jaspsi CCUG 53607 (AB264131)94.20PositiveND
S. echinoides CCUG 2870 (AB021370)94.13PositiveND
M. oculi strain SB1-30.5M. oculi CCUG 43427AT (FR773700)98.94
M. timonae CCUG 45783 (U54470)97.14Negative0.25
  • a ND, not determined, as the strain was not available or did not grow on Mueller-Hinton medium.